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Image Search Results
Journal: PLoS ONE
Article Title: Survival and death of intestinal cells infected by Chlamydia trachomatis
doi: 10.1371/journal.pone.0215956
Figure Lengend Snippet: Cytofluorimetric analysis of Annexin V/propidium iodide double staining of cell lines infected for 72 h with CT serovars D and L2 at MOI 3. FITC-A channel (x-axis) is used for the detection of Annexin V-EGFP fluorescence. PE-A channel (y-axis) is used for the detection of propidium iodide fluorescence. Data are representative of three independent experiments each performed in triplicate.
Article Snippet: After 24 or 72 h, cells were trypsinized, centrifuged at 400 × g for 5 min, washed in 1 ml of 0.14 M NaCl, 5 mM NaH 2 PO 4 , pH 7 (PBS), centrifuged again in cytofluorimeter tubes (Falcon, Franklin Lakes, NJ, USA) and re-suspended in 294 μL binding buffer provided in the
Techniques: Double Staining, Infection, Fluorescence